Efficiently smuggling drugs into cells — ScienceDaily


A new patented method called Progressive Mechanoporation makes it possible to mechanically disrupt the membranes of cells for a short time period and let drugs or genes inside cells. In this way, researchers can test new therapies more easily than before. Modern vaccines such as those against Sars-CoV-2 use tiny lipid spheres to transport genetic […]

Gold leaf could help diagnose viral infections in low-resource settings — ScienceDaily


Gold leaf — gold metal hammered into thin sheets — is used by artists and crafters to gild picture frames, artwork and clothing. Despite its luxurious appearance, the material is affordable and available at most craft stores. Now, researchers reporting in ACS Central Science have developed gold leaf electrodes that, in combination with a CRISPR-based […]

Proof-of-principle research shows that genes can be accurately edited in cells throughout the body — ScienceDaily


Researchers at the University of California San Diego have laid the groundwork for a potential new type of gene therapy using novel CRISPR-based techniques. Working in fruit flies and human cells, research led by UC San Diego Postdoctoral Scholar Zhiqian Li in Division of Biological Sciences Professor Ethan Bier’s laboratory demonstrates that new DNA repair […]

Blind patients hope to gain vision in medical first of CRISPR editing DNA inside their bodies


In a world first, scientists are using the controversial CRISPR technology to edit genes while the DNA is still inside a person’s body. Typically, the tool –  used to treat conditions like sickle cell disease – removes cells from the body, edits out the mutated parts and then reinserts them, along with instructions to create […]

Researchers speed identification of DNA regions that regulate gene expression — ScienceDaily


St. Jude Children’s Research Hospital scientists have developed an integrated, high-throughput system to better understand and possibly manipulate gene expression for treatment of disorders such as sickle cell disease and beta thalassemia. The research appears today in the journal Nature Genetics. Researchers used the system to identify dozens of DNA regulatory elements that act together […]

New gene editing technique enables millions of genetic experiments to be performed simultaneously — ScienceDaily


Researchers have created a new gene editing tool called Retron Library Recombineering (RLR) that can generate up to millions of mutations simultaneously, and ‘barcodes’ mutant bacterial cells so that the entire pool can be screened at once. It can be used in contexts where CRISPR is toxic or not feasible, and results in better editing […]

New genetic target for blood cancer treatment — ScienceDaily


Targeting a pathway that is essential for the survival of certain types of acute myeloid leukaemia could provide a new therapy avenue for patients, the latest research has found. Researchers from the Wellcome Sanger Institute found that a specific genetic mutation, which is linked with poor prognosis in blood cancer, is involved in the development […]

Single-cell CRISPR technology deciphers role of chromatin accessibility in cancer — ScienceDaily


In a new resource for the scientific community, published today in Nature Biotechnology, researchers in the lab of Neville Sanjana, PhD, at the New York Genome Center (NYGC) and New York University (NYU) developed CRISPR-sciATAC, a novel integrative genetic screening platform that jointly captures CRISPR gene perturbations and single-cell chromatin accessibility genome-wide. With this technology, […]

New approach to gene therapy can correct any disease-causing mutation within a gene — ScienceDaily


A new approach to gene editing using the CRISPR/Cas9 system bypasses disease-causing mutations in a gene, enabling treatment of genetic diseases linked to a single gene, such as cystic fibrosis, certain types of sickle cell anemia, and other rare diseases. The method, developed and tested in mice and human tissue cultures by researchers at Penn […]

Chickens and pigs with integrated genetic scissors — ScienceDaily


Researchers at the TUM have demonstrated a way to efficiently study molecular mechanisms of disease resistance or biomedical issues in farm animals. Researchers are now able to introduce specific gene mutations into a desired organ or even correct existing genes without creating new animal models for each target gene. This reduces the number of animals […]

New ‘time machine’ technique to measure cells — ScienceDaily


Using a new single-cell technique, WEHI researchers have uncovered a way to understand the programming behind how stem cells make particular cell types. The research uncovered 30 new genes that program stem cells to make the dendritic cells that kick-start the immune response. By uncovering this process, the researchers hope they will be able to […]

New CRISPR technology offers unrivaled control of epigenetic inheritance — ScienceDaily


Scientists have figured out how to modify CRISPR’s basic architecture to extend its reach beyond the genome and into what’s known as the epigenome — proteins and small molecules that latch onto DNA and control when and where genes are switched on or off. In a paper published April 9, 2021, in the journal Cell, […]

Genetically modifying mosquitoes to express antimalarial genes and pass them on to their offspring is being tested as a new strategy to eliminate malaria — ScienceDaily


Altering a mosquito’s gut genes to make them spread antimalarial genes to the next generation of their species shows promise as an approach to curb malaria, suggests a preliminary study published today in eLife. The study is the latest in a series of steps toward using CRISPR-Cas9 gene-editing technology to make changes in mosquito genes […]

Toward a reliable oral treatment for sickle cell disease — ScienceDaily


For the millions of people worldwide who have sickle cell disease, there are only a few treatment options, which include risky bone marrow transplants, gene therapy or other treatments that address a subset of symptoms. Today, researchers will describe the discovery of a small molecule with the potential to address the root cause of sickle […]

PNA-based technique an essential part of the gene editing toolkit — ScienceDaily


In an article published in the April 8 issue of Nature, the National Institutes of Health’s Somatic Cell Gene Editing Consortium provided a detailed update on the progress of their nationwide effort to develop safer and more effective methods to edit the genomes of disease-relevant somatic cells and reduce the burden of disease caused by […]

Katër ndodhi të jashtëzakonshme shkencore të 2020-s, të cilat ndoshta s’ i dini për shkak të virusit


Viti që lamë pas ishte një vit i trazuar për shkak të pandemisë. Megjithatë, nga një tjetër këndvështrim shkencor, ishte një vit përparimesh të jashtëzakonshme 1. Viti i kodit gjenetik Redaktimi i gjeneve është një nga dukuritë më të rëndësishme shkencore të vitit që lamë pas. Në tetor, për herë të parë në histori, një […]

Forschungsgruppe entdeckt für Biodiversität verantwortliches Gen


Die Arbeitsgruppe von Christiane Nüsslein-Volhard am Max-Planck-Institut für Entwicklungsbiologie in Tübingen hat ein Gen identifiziert, das für die unterschiedlichen Muster von nahverwandten Arten des Zebrabärblings Danio rerio mitverantwortlich ist. Das Gen, Obelix, codiert für einen Kaliumkanal; diese Klasse von Proteinen steuert zahlreiche zelluläre Prozesse, die in einigen menschlichen Krankheiten gestört sind. Ihre Ergebnisse haben sie […]

Gene-editing treatment shows promise for sickle cell disease


This July 2019 image provided by the Sarah Cannon Research Institute shows Victoria Gray on her infusion day during a gene editing trial for sickle cell disease at the Sarah Cannon Research Institute and The Children’s Hospital At TriStar Centennial in Nashville. Since her treatment, Gray has weaned herself from pain medications she depended on […]

Mimicking effect of exercise with gene therapy


Gene therapy research can benefit people who have had accidents, who are in wheelchairs, or who have diseases that prevent the possibility of physical expression – but also people who generally respond poorly to training. Credit: Scanpix / Shutterstock It sounds too good to be true—and it is. But Jose Bianco Moreira and the CERG […]

New immunotherapeutic approach takes aim at cancer’s enzyme shield


Credit: Brigham and Women’s Hospital Immunotherapies have transformed the landscape of cancer treatment by allowing physicians to alter or augment patients’ immune response to better attack malignant tumors. But some tumors—known as “cold tumors”—fly under the immune system’s radar. Checkpoint inhibitors, which are the basis of many immunotherapies, don’t work well against them. A team […]

Team discovers new connection between Alzheimer’s dementia and Dlgap2


Andrew Ouellette and Sarah Neuner. Credit: Tiffany Laufer A gene known for helping facilitate communication between neurons in the nervous system has been discovered to be connected with Alzheimer’s dementia and cognitive decline, according to a national research team led by The Jackson Laboratory and University of Maine. Catherine Kaczorowski, associate professor and Evnin family […]

Engineered immune cells elicit broad response to HIV in mice, offering hope for vaccine


Antibodies from engineered B cells approach an HIV particle (shown in blue) to disable it. In a new study, scientists found that gene-edited B cells can generate durable protective antibody responses against HIV when activated with a vaccine. Credit: Illustration courtesy of the Voss laboratory at Scripps Research. Unlike so many other deadly viruses, HIV […]

Revolutionary CRISPR-based genome editing system treatment destroys cancer cells


Cancer cell during cell division. Credit: National Institutes of Health Researchers at Tel Aviv University (TAU) have demonstrated that the CRISPR/Cas9 system is very effective in treating metastatic cancers, a significant step on the way to finding a cure for cancer. The researchers developed a novel lipid nanoparticle-based delivery system that specifically targets cancer cells […]

Erste hochskalierbare Methode zur Untersuchung der Proteinlevel und Proteinlokalisierungen in Zellen entwickelt


Forscher des CeMM haben eine hochskalierbare Methode entwickelt, die es erlaubt, Hunderte von Proteinen parallel zu untersuchen, um die Veränderungen ihrer Mengen und Lokalisierungen in der Zelle zu verfolgen. Diese neuartige Strategie kann beispielsweise verwendet werden, um die Auswirkungen von Wirkstoffkandidaten auf zahlreiche Proteine in der Zelle zu beobachten und stellt somit einen wesentlichen Beitrag […]

Induced liver regeneration enhances CRISPR/Cas9-mediated gene repair


Credit: CC0 Public Domain Use of thyroid hormone to boost hepatocyte proliferation enhanced the efficiency of CRISPR/Cas9-mediated gene correction in the mouse liver. This dietary induction of hepatocyte regeneration may be a viable clinical strategy to enhance gene repair in the liver, according to the peer-reviewed journal Human Gene Therapy. The study was done in […]

Understanding rare type of diabetes gives insights into mechanisms of insulin production


Credit: CC0 Public Domain Solving the genetic puzzle of why babies developed a rare type of diabetes has uncovered a new biological pathway that is fundamental to insulin production, and could boost research into new treatments for more common forms of diabetes. Published today (9th November) in the Journal of Clinical Investigation, the study used […]

Shining a light on the role of the genome’s ‘dark matter’ in cancer development


CRISPRi screens to identify functional Wnt-regulated lncRNAs that are important for pancreatic cancer growth in different models. Credit: Excerpted from Figure 4 in Liu, S., Harmston, N., Glaser, T.L. et al. Wnt-regulated lncRNA discovery enhanced by in vivo identification and CRISPRi functional validation. Genome Med 12, 89 (2020). doi:10.1186/s13073-020-00788-5, under a Creative Commons Attribution 4.0 […]

Crispr Gene Editing Can Cause Unwanted Changes in Human Embryos, Study Finds


In 2017, a team of researchers led by Shoukhrat Mitalipov, a geneticist at Oregon Health and Science University in Portland, reported that human embryos carrying a mutation could be coaxed into this process without a synthetic template. The researchers generated embryos from a union between two cells: a sperm carrying a mutation that can make […]

Cancer-fighting gene restrains ‘jumping genes’


This illustration depicts how retrotransposons are “handcuffed” by the tumor suppressor gene p53. But when p53 is lost, these mobile elements can erupt. Credit: Study authors Amanda Jones and Bhavana Tiwari. Artwork by Angela Diehl. About half of all tumors have mutations of the gene p53, normally responsible for warding off cancer. Now, UT Southwestern […]

New COVID-19 related genes—helpful and harmful—found in massive screen


3D print of a spike protein of SARS-CoV-2, the virus that causes COVID-19–in front of a 3D print of a SARS-CoV-2 virus particle. The spike protein (foreground) enables the virus to enter and infect human cells. On the virus model, the virus surface (blue) is covered with spike proteins (red) that enable the virus to […]

Kohlmotte nutzt Pflanzenabwehrstoff als Signal für die Eiablage


Ein Forschungsteam konnte zeigen, dass Isothiocyanate, die Kreuzblütengewächse eigentlich zur Verteidigung gegen Fraßfeinde bilden, Kohlmottenweibchen als Duftsignale dienen, damit sie ihre Eier auf diesen Pflanzen ablegen können. Die Wissenschaftler identifizierten zwei Geruchsrezeptoren, deren einzige Aufgabe darin besteht, diese Duftstoffe aufzuspüren und den Weg zum idealen Eiablageplatz zu weisen. Kreuzblütengewächse, wie Kohl, Raps, Senf und Meerrettich, […]

Study reveals restoration of retinal and visual function following gene therapy


A mutation in an inherited blindness mouse model abolishes the expression of RPE65, a key enzyme in a visual cycle, in the RPE cells. Base editing treatment can correct the mutation and restore functional RPE65 (green), thereby restoring vision in mice. Credit: Nature Biomedical Engineering A breakthrough study, led by researchers from the University of […]

Single gene disorders not so simple after all


Credit: CC0 Public Domain Traditionally, geneticists divide disorders into “simple,” where a single gene mutation causes disease, or complex, where mutations in many genes contribute modest amounts. A new study suggests that the truth is somewhere in between. For many years, scientists studying patient genomes have gained glimpses of genetic “burden” or additional genetic variation […]

Fituesja e Nobelit në Kimi krijon testin që zbulon virusin në pesë minuta: Perspektiva e saj për COVID


Një grup studiuesish me bazë në Kaliforni kanë zhvilluar një test që zbulon virusin në pesë minuta, duke përdorur teknologjinë e ndryshimit të gjeneve si edhe aparatet fotografike të modifikuara në telefonat celularë, një metodë kjo që mund të ndihmojë shumë në luftimin e pandemisë Studimi, i udhëhequr nga Dr. Jennifer Doudna, një nga fitueset […]

What’s Special About Bat Viruses? What We Don’t Know Could Hurt Us


Bats were once of interest mainly to specialists and devoted conservationists. But the global pandemic pushed the animals squarely into the spotlight as the apparent original source of the novel coronavirus. Now, once arcane research into the large number of viruses that live in bats has acquired a new urgency, along with discussions of what […]

Eine Schere für Gene – Biermann Medizin


Nobelpreis an Team um Berliner Forscherin Es gibt Momente in der Forschungsgeschichte, die alles verändern – mit der Genschere Crispr/Cas9 ist ein solcher Coup gelungen. Immens schnell verbreitete sich die Methode in Laboren weltweit. Nun gibt es für die Entwicklung den Nobelpreis. Grund zum Jubeln auch in Berlin. Revolution, Wunderwerkzeug, Siegeszug – wohl keine andere […]

US-French duo win Nobel Chemistry Prize for gene editing tool


Nobel Chemistry prize winners Emmanuelle Charpentier (L) and Jennifer Doudna of the United States Emmanuelle Charpentier of France and Jennifer Doudna of the US on Wednesday won the Nobel Chemistry Prize for the gene-editing technique known as the CRISPR-Cas9 DNA snipping “scissors”, the first time a Nobel science prize has gone to a women-only team. […]

Chemie-Nobelpreis geht an die Genforscherinnen Charpentier und Doudna


Büste Alfred Nobel in Geesthacht. Foto: Arndt, Friedberg stock.adobe.com Der Nobelpreis für Chemie geht in diesem Jahr an die derzeit in Deutschland arbeitende Genforscherin Emmanuelle Charpentier (Frankreich) sowie an Jennifer A. Doudna (USA) für die Entwicklung von Methoden zur Erbgut-Veränderung. Das teilte die Königlich-Schwedische Akademie der Wissenschaften am Mittwoch in Stockholm mit. Sie haben die […]

Emmanuelle Charpentier, Jennifer Doudna win Nobel Prize for chemistry for genome editing


Issued on: 07/10/2020 – 12:04 French scientist Emmanuelle Charpentier and American Jennifer A. Doudna have won the Nobel Prize in chemistry for developing a method of genome editing known as CRISPR. The recipients were announced Wednesday in Stockholm by Goran Hansson, Secretary General of the Royal Swedish Academy of Sciences. The prestigious award comes with […]

Promising COVID-19 rapid test technology enters phase 1 of NIH challenge


Close-up of early Rover photothermal PCR prototype, a promising new COVID-19 rapid-testing technology platform developed by Rover Diagnostics and Columbia Engineering that has been selected by the NIH to enter Phase 1 of the RADx initiative to support new COVID-19 testing technologies. Credit: Bulent Onalir/Rover Diagnostics A promising new COVID-19 rapid-testing technology platform developed by […]

A genetic variant that protects against Alzheimer’s disease promotes immune cell functions


Credit: Unsplash/CC0 Public Domain A new study conducted by researchers at the University of Eastern Finland found that the PLCG2-P522R genetic variant, which protects against Alzheimer’s disease, enhances several key functions of immune cells. The results obtained in the study highlight the importance of immune cells as a target of future development of new therapies […]