New gene editing technique enables millions of genetic experiments to be performed simultaneously — ScienceDaily

Researchers have created a new gene editing tool called Retron Library Recombineering (RLR) that can generate up to millions of mutations simultaneously, and ‘barcodes’ mutant bacterial cells so that the entire pool can be screened at once. It can be used in contexts where CRISPR is toxic or not feasible, and results in better editing […]

A new strain of a well-known probiotic might offer help for infants’ intestinal problems — ScienceDaily

Lacticaseibacillus rhamnosus GG, or LGG, is the most studied probiotic bacterium in the world. However, its features are not perfect, as it is unable to utilise the milk carbohydrate lactose or break down the milk protein casein. This is why the bacterium grows poorly in milk and why it has to be separately added to […]

Single-cell CRISPR technology deciphers role of chromatin accessibility in cancer — ScienceDaily

In a new resource for the scientific community, published today in Nature Biotechnology, researchers in the lab of Neville Sanjana, PhD, at the New York Genome Center (NYGC) and New York University (NYU) developed CRISPR-sciATAC, a novel integrative genetic screening platform that jointly captures CRISPR gene perturbations and single-cell chromatin accessibility genome-wide. With this technology, […]

New approach to gene therapy can correct any disease-causing mutation within a gene — ScienceDaily

A new approach to gene editing using the CRISPR/Cas9 system bypasses disease-causing mutations in a gene, enabling treatment of genetic diseases linked to a single gene, such as cystic fibrosis, certain types of sickle cell anemia, and other rare diseases. The method, developed and tested in mice and human tissue cultures by researchers at Penn […]

PNA-based technique an essential part of the gene editing toolkit — ScienceDaily

In an article published in the April 8 issue of Nature, the National Institutes of Health’s Somatic Cell Gene Editing Consortium provided a detailed update on the progress of their nationwide effort to develop safer and more effective methods to edit the genomes of disease-relevant somatic cells and reduce the burden of disease caused by […]

Gene-editing treatment shows promise for sickle cell disease

This July 2019 image provided by the Sarah Cannon Research Institute shows Victoria Gray on her infusion day during a gene editing trial for sickle cell disease at the Sarah Cannon Research Institute and The Children’s Hospital At TriStar Centennial in Nashville. Since her treatment, Gray has weaned herself from pain medications she depended on […]

Mimicking effect of exercise with gene therapy

Gene therapy research can benefit people who have had accidents, who are in wheelchairs, or who have diseases that prevent the possibility of physical expression – but also people who generally respond poorly to training. Credit: Scanpix / Shutterstock It sounds too good to be true—and it is. But Jose Bianco Moreira and the CERG […]

Induced liver regeneration enhances CRISPR/Cas9-mediated gene repair

Credit: CC0 Public Domain Use of thyroid hormone to boost hepatocyte proliferation enhanced the efficiency of CRISPR/Cas9-mediated gene correction in the mouse liver. This dietary induction of hepatocyte regeneration may be a viable clinical strategy to enhance gene repair in the liver, according to the peer-reviewed journal Human Gene Therapy. The study was done in […]

Understanding rare type of diabetes gives insights into mechanisms of insulin production

Credit: CC0 Public Domain Solving the genetic puzzle of why babies developed a rare type of diabetes has uncovered a new biological pathway that is fundamental to insulin production, and could boost research into new treatments for more common forms of diabetes. Published today (9th November) in the Journal of Clinical Investigation, the study used […]

Shining a light on the role of the genome’s ‘dark matter’ in cancer development

CRISPRi screens to identify functional Wnt-regulated lncRNAs that are important for pancreatic cancer growth in different models. Credit: Excerpted from Figure 4 in Liu, S., Harmston, N., Glaser, T.L. et al. Wnt-regulated lncRNA discovery enhanced by in vivo identification and CRISPRi functional validation. Genome Med 12, 89 (2020). doi:10.1186/s13073-020-00788-5, under a Creative Commons Attribution 4.0 […]

Crispr Gene Editing Can Cause Unwanted Changes in Human Embryos, Study Finds

In 2017, a team of researchers led by Shoukhrat Mitalipov, a geneticist at Oregon Health and Science University in Portland, reported that human embryos carrying a mutation could be coaxed into this process without a synthetic template. The researchers generated embryos from a union between two cells: a sperm carrying a mutation that can make […]

Cancer-fighting gene restrains ‘jumping genes’

This illustration depicts how retrotransposons are “handcuffed” by the tumor suppressor gene p53. But when p53 is lost, these mobile elements can erupt. Credit: Study authors Amanda Jones and Bhavana Tiwari. Artwork by Angela Diehl. About half of all tumors have mutations of the gene p53, normally responsible for warding off cancer. Now, UT Southwestern […]

Disputes over when life begins may block cutting-edge therapies like mitochondrial replacement

Credit: CC0 Public Domain The nomination of Judge Amy Coney Barrett to the U.S. Supreme Court has once again pushed the debate over when life begins into the headlines, which could have far-reaching effects on access to both current and emerging reproductive technologies. In 2006, Judge Barrett was one of the signatories on a newspaper […]

Single gene disorders not so simple after all

Credit: CC0 Public Domain Traditionally, geneticists divide disorders into “simple,” where a single gene mutation causes disease, or complex, where mutations in many genes contribute modest amounts. A new study suggests that the truth is somewhere in between. For many years, scientists studying patient genomes have gained glimpses of genetic “burden” or additional genetic variation […]

US-French duo win Nobel Chemistry Prize for gene editing tool

Nobel Chemistry prize winners Emmanuelle Charpentier (L) and Jennifer Doudna of the United States Emmanuelle Charpentier of France and Jennifer Doudna of the US on Wednesday won the Nobel Chemistry Prize for the gene-editing technique known as the CRISPR-Cas9 DNA snipping “scissors”, the first time a Nobel science prize has gone to a women-only team. […]

A genetic variant that protects against Alzheimer’s disease promotes immune cell functions

Credit: Unsplash/CC0 Public Domain A new study conducted by researchers at the University of Eastern Finland found that the PLCG2-P522R genetic variant, which protects against Alzheimer’s disease, enhances several key functions of immune cells. The results obtained in the study highlight the importance of immune cells as a target of future development of new therapies […]